Which class of direct-acting antivirals for hepatitis C inhibits the formation of the membranous web for viral replication?

The correct answer is that NS5A replication complex inhibitors play a critical role in inhibiting the formation of the membranous web, which is essential for the replication of the hepatitis C virus (HCV). These inhibitors specifically target the NS5A protein, which is involved in viral RNA replication and assembly. By disrupting the function of NS5A, these medications prevent the structural changes necessary for the virus to replicate effectively within the host cell, thereby inhibiting viral propagation.

In contrast, other classes of antiviral drugs serve different functions. NS3/NS4A protease inhibitors primarily inhibit viral protease activity, preventing the cleavage of viral polyproteins and subsequent maturation of the virus. NS5B polymerase inhibitors target the viral polymerase enzyme, blocking RNA synthesis but not necessarily affecting the membranous web structure directly. Interferons, on the other hand, enhance the host's immune response against the virus but do not have a specific mechanism related to the membranous web formation. Thus, NS5A replication complex inhibitors are unique in their targeted approach to inhibit the specific structures required for HCV replication.